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Today's discussion is about the dot blot hybridization technique. It is used to detect specific DNA or RNA sequences in samples through hybridization. Southern blotting is used for known fragments, while northern blotting is used for new fragments. The probe binds to the target DNA forming a double-stranded DNA, making it easy to identify. Steps in the technique include purifying DNA or RNA samples, applying them to a nitrocellulose or nylon membrane, denaturing the DNA, and fixing it. Nitrocellulose membranes are preferred for their affinity for nucleic acids.
