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hello everyone i am dr niraj and today i will discuss about dot blot and slot blot hybridization so without any delay lets move to the video what is dot blot and slot plot so these are the techniques which are used to identify a particular dna rna or protein from the sample we can also quantify or we can also determine the concentration of these molecules by using these techniques and these techniques that do not require any kind of restriction digestion and electrophoresis means there is no need to perform electrophoresis and the digestion we can directly apply the sample and detect a particular dna rna protein from that sample what we actually did in these techniques we just apply or you can see we just bloat our sample to the membrane and ultimately detect a particular molecule from these samples and the name given to this technique on the basis of the shape of the you can say the sample the shape in which we load or we blot the sample on the membrane like in case of dot blot so t