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Today we discuss the dotplot hybridization technique, used to detect specific DNA or RNA sequences in electrophoresis samples. Southern blotting is for known fragments, northern blotting for new ones. The probe binds to the target DNA, forming double-stranded DNA. Steps: purify DNA/RNA, use nitrocellulose or nylon membrane, apply samples, denature DNA, and fix it. Ultimately, the probe's radio label helps identify it easily.
