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welcome to biology exam sorry calm and this tutorial we are going to discuss about different plotting techniques definition and also some major important points according to central dogma of biology achene is a segment of DNA that codes for a functional polypeptide in a molecular biology lab we may be looking at a specific DNA for expression studies we may be looking at an mRNA or a polypeptide the technique to find out a specific DNA in a sample is called as a southern blotting but as in for expression studies we may look either at mRNA or protein the technique to find out specific mRNA is called as a northern blotting and also in the case of a protein the technique to find out a specific protein in a sample is called as a Western blotting so well be looking into some aspects of southern blotting the technique was developed by e/m southern in 1975 its a technique used to detect the presence of a particular DNA fragment in a sample the principle of this technique is hybridization we