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Dot blot is a method used to detect proteins or nucleic acids by directly spotting samples onto a membrane for probing. It is similar to Western and Southern blotting but simpler, making it useful for monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency with biotin, the sample is compared to a biotinylated reference standard using serial dilutions. A 10-fold dilution range is recommended for accuracy.