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Dot blot is a quick and simple method for detecting biological samples like proteins or nucleic acids. It is similar to western blotting and southern blotting but involves directly dotting samples onto a membrane for detection. This method is used for various applications such as monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency with biotin, compare the labeled sample to a known biotinylated reference standard by making serial dilutions of both in the same manner. A 10-fold serial dilution is recommended for a wide range of samples.