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Dot blot is a quick and easy method for detecting biological samples like proteins or nucleic acids, similar to western blotting and southern blotting. Samples are dotted onto a membrane and probed for detection. It is used for various applications such as monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency with biotin, compare the labeled sample to a known biotinylated reference standard through serial dilutions.
