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imagine that youamp;#39;re only interested in one specific protein and trying to detect its presence across hundreds of samples each sample is from a different batch of cells and there are bound to be other proteins or mixtures of contaminants that will limit your ability to detect the protein of interest the western bologna technique is a sophisticated method of protein analysis as compared to a more global detection method like canassi staining both techniques can be used to estimate protein size when coupled with sds page but vary in their specificity and sensitivity in principle the western blot is very similar to other immunological assays that rely on antibody binding to detect proteins these assays are robust as the quality of the antibiotics available and these material requirements can be quite expensive compared to other standing methods like the camassi stain the western blot is very sensitive and very specific depending on the antibodies used this technique can det
