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good day and welcome to todayamp;#39;s practical course video tutorial in this video tutorial we will learn how to perform protein immunoblock also known as western blood protein immunoblot is a sensitive assay for the detection and characterization of protein through antigen antibody recognition it is performed by immobilizing protein and remembering and detecting the protein using an antibody the name western blot is a play of names because at first a technique for dna detection by blotting was earlier developed by professor edwin southen and as such was referred to as southern blood technique for dna detection subsequently another similar blood technique was used to detect rna and was codenamed northern blood hence when immunoblot protein detection was developed it was also codenamed western blood to follow the initial southern and northern blotting techniques sdsp detects the presence and the molar mass of proteins after sdsp gelato fluoresces protein bonds become visible
