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hello friends welcome to bmh learning this video is about biotin strepta between labeling the biotin streptavidin complex is one of the most popular tagging systems for the conjugation of biomolecules the widespread adoption of the biotin streptavidin conjugation system is primarily due to two factors the first is the relatively small size of the biotin and streptavidin molecules themselves which allows for extensive binding to biologically active macromolecules such as antibodies without impedance to their functions the second is the specificity with which biotin and streptavidin bind to each other this in large part is due to the tetrameric confirmation of streptavidin once streptavidin protein has the capacity to bind to four biotin molecules with high affinity and selectivity this multiplicity enables the amplification of big signals streptavidin coated surfaces are characterized by very low non-specific bindings the streptavidin tetramers have an extraordinarily high binding affin